Myotube apoptotic evaluation.

Photomicrographs of TUNEL assay (green staining) showing myotubes treated with hydrogen peroxide—H2O2 (A-C), untreated control myotubes—CTR (D-F), myotubes pre-incubated with carnosinol (30 mM) followed by hydrogen peroxide treatment—CNS + H2O2 (G-I), pre-incubated with carnosine (30 mM) followed by hydrogen peroxide treatment—CAR + H2O2 (J-L) and pre-incubated with anserine (30 mM) followed by hydrogen peroxide treatment—ANS + H2O2 (M-O). DAPI (blue staining) is used to locate the nuclei of the cells. Bar equal: 20 μm. The graphs (P and Q) summarize the quantitative analysis of TUNEL positive myotubes of all the experimental cell groups. * p≤0.05 vs CTR; # p≤0.05 vs H2O2; § p≤0.05 vs CAR + H2O2 10 mM; §§ p≤0.05 vs CAR + H2O2 20 mM; §§§ p≤0.05 vs CAR + H2O2 30 mM; + p≤0.05 vs ANS + H2O2 10 mM; ++ p≤0.05 vs ANS + H2O2 20 mM and +++ p≤0.05 vs ANS + H2O2 30 mM.