Coelenterazine (CTZ)-utilizing marine luciferases and their derivatives have attracted significant attention because of their ATP-independency, fast enzymatic turnover, and high bioluminescence brightness. However, marine luciferases typically emit blue photons and their substrates, including CTZ and the recently developed diphenylterazine (DTZ), have poor water solubility, hindering their in vivo applications. Herein, we report a family of pyridyl CTZ and DTZ analogs that exhibit spectrally shifted emission and improved water solubility. Through directed evolution, we engineered a LumiLuc luciferase with broad substrate specificity. In the presence of corresponding pyridyl substrates (i.e., pyCTZ, 6pyDTZ, or 8pyDTZ), LumiLuc generates high...
Bioluminescence imaging with luciferase-luciferin pairs is commonly used for monitoring biological p...
The functioning of bioluminescent systems in most of the known marine organisms is based on the oxid...
In vivo bioluminescent imaging using cells expressing Renilla luciferase is becoming increasingly co...
Coelenterazine (CTZ)-utilizing marine luciferases and their derivatives have attracted significant a...
Red-shifted bioluminescence reporters are desirable for biological imaging. We describe the developm...
A unique combinatorial bioluminescence (BL) imaging system was developed for determining molecular e...
International audienceWe describe here an extensive structure-bioluminescence relationship study of ...
Optical reporters have revolutionized our ability to visualize biological mechanisms in action. Amon...
Native coelenterazine (nCTZ) is a common substrate to most marine luciferases and photoproteins. In ...
Bioluminescence imaging is widely used for cell-based assays and animal imaging studies in biomedica...
Bioluminescence is widely used for real-time imaging in living organisms. This technology features a...
Bioluminescence imaging with luciferase-luciferin pairs is routinely used to monitor cellular events...
Bioluminescence imaging with luciferase-luciferin pairs is routinely used to monitor cellular functi...
Firefly luciferases, which emit visible light in a highly specific ATP-dependent process, have been ...
Genetically-encoded fluorescent probes have revolutionized our understanding of biological systems. ...
Bioluminescence imaging with luciferase-luciferin pairs is commonly used for monitoring biological p...
The functioning of bioluminescent systems in most of the known marine organisms is based on the oxid...
In vivo bioluminescent imaging using cells expressing Renilla luciferase is becoming increasingly co...
Coelenterazine (CTZ)-utilizing marine luciferases and their derivatives have attracted significant a...
Red-shifted bioluminescence reporters are desirable for biological imaging. We describe the developm...
A unique combinatorial bioluminescence (BL) imaging system was developed for determining molecular e...
International audienceWe describe here an extensive structure-bioluminescence relationship study of ...
Optical reporters have revolutionized our ability to visualize biological mechanisms in action. Amon...
Native coelenterazine (nCTZ) is a common substrate to most marine luciferases and photoproteins. In ...
Bioluminescence imaging is widely used for cell-based assays and animal imaging studies in biomedica...
Bioluminescence is widely used for real-time imaging in living organisms. This technology features a...
Bioluminescence imaging with luciferase-luciferin pairs is routinely used to monitor cellular events...
Bioluminescence imaging with luciferase-luciferin pairs is routinely used to monitor cellular functi...
Firefly luciferases, which emit visible light in a highly specific ATP-dependent process, have been ...
Genetically-encoded fluorescent probes have revolutionized our understanding of biological systems. ...
Bioluminescence imaging with luciferase-luciferin pairs is commonly used for monitoring biological p...
The functioning of bioluminescent systems in most of the known marine organisms is based on the oxid...
In vivo bioluminescent imaging using cells expressing Renilla luciferase is becoming increasingly co...