Performance of the assembled fluorescence microscope.

(a) MTF plot calculated for the image plane in both the tangential and sagittal directions showing the nominal performance of the system. (b) Image taken with a 455 nm LED of a high resolution 1951 USAF target presenting the measured performance of the system. The smallest resolvable feature on the target was group 8 element 1 (shown enlarged and outlined in white), demonstrated by the intensity profiles through the vertical and horizontal elements indicated by the red lines and arrows. Resolution target image has been contrast enhanced for visualization purposes.