Fine-mapping of the Ma2 QTL.

The Ma2 QTL spans from 67.3 Mpb to 69.1 Mbp (light blue bar). Five F2 individuals that were heterozygous across the Ma2 QTL were self-pollinated to generate heterogeneous inbred families (HIFs) totaling 1000 F3 individuals. Genotype and phenotype analysis of these HIFs narrowed the QTL region to ~600 kb (darker blue bar). Two additional rounds of fine-mapping narrowed the QTL region to ~500 kb (vertical dashed lines). This region contained 76 genes. The genotypes of relevant HIFs and the parents are shown to the left and their corresponding days to flowering are shown to the right. Blue regions correspond to the 80M genotype and red regions correspond to the Hegari genotype. Purple regions are heterozygous.