Additional file 3: of CD73 promotes hepatocellular carcinoma progression and metastasis via activating PI3K/AKT signaling by inducing Rap1-mediated membrane localization of P110β and predicts poor prognosis

Figure S2. Function of CD73 in Hep3B cell line. (A) Efficiencies of CD73 knockdown in Hep3B cells were evaluated by WB assays. (B) Effects of CD73 knockdown on proliferation in Hep3B cells were evaluated by CCK-8 and colony formation assays. (C) Effects of CD73 knockdown on cell cycle in Hep3B cells were evaluated by flow cytometry assays. (D) Effects of CD73 knockdown on apoptosis in Hep3B cells were evaluated by flow cytometry assays. (E) Effects of CD73 knockdown on migration and invasion in Hep3B cells were evaluated by Transwell assays. (F) Effects of CD73 knockdown on migration were validated by wound healing assays. (G) Effects of CD73 knockdown on in vivo tumor growth. “N.S.” indicated not significant; Asterisk indicated P < 0.050, all in vitro experiments were performed in triplicate, t tests were used. (TIF 10457 kb