Additional file 1: of GPR34 in spinal microglia exacerbates neuropathic pain in mice

Figure S1. Microglial activation does not occur in control groups. Dorsal horn tissues at the L4 level were obtained 7 days after injury (n = 4), and mRNA expression of a microglial marker, Iba1 (a), and a prominently upregulated cytokine at day 7, IL-1β (b), was analyzed by qRT-PCR. Results are normalized to GAPDH and shown as fold change over naive sample. Expression of both molecules in sham-operated mice (Sham) and the contralateral side of operated mice (Contra) was equivalent to that in non-operated mice (Naive). In contrast, expression of both molecules was significantly increased in the ipsilateral side of operated mice (Ipsi). Values are mean ± SEM. **p < 0.01, ***p < 0.001 (one-way ANOVA with post hoc Turkey’s test). (DOCX 50 kb