Insulin restores abnormal follicle development by regulates the PI3K/AKT signaling pathway in newborn mouse ovaries.

(A-F) Real-time PCR analysis of mRNA expression levels of Nobox, Figla, Sohlh2, PI3K and Insulin and Receptor in STZ-treated and Insulin-STZ-treated groups. (G-H) The expression of PI3K protein was analyzed by western blot. Expression of β-actin was used as a loading control. At least 5 mice were analyzed in each group; Date are presented as mean ± SEM. * shows a significant difference between STZ-treated and control at P < 0.05; ** shows a significant difference at P < 0.01.