Nuclear import of viral genomes in different A549 cell lines.

Model fit (lines) to experimental data (circles ± standard deviation, n = 4) for the import of viral genomes (vRNPs) in cycloheximide-treated cell lines upon infection by A/PR/8/34 (H1N1) at MOI 50. Relative increase in fluorescence intensity (FI) of the nucleus was determined by imaging flow cytometry after co-staining of cells with DAPI and vRNP antibody. The nuclear import rate was estimated by fitting the simulated fraction of nuclear vRNPs to the averaged experimental data. To account for the background signal of the nucleus in images, an offset of approximately 50% at 0 h p.i. was applied with respect to the experimental data obtained for parental A549 cells (A-F, blue), the transduction control (A, brown) and engineered cell lines overexpressing one of the following host cell factors: CEACAM6 (B, brown), FANCG (C, brown), NXF1 (D, brown), PLD2 (E, brown), XAB2 (F, brown). The asterisks indicate differences in relative FI levels with respect to the parental A549 cell line, that were either noticeable, however, statistically not significant (* p ≤ 0.1), or significant (** p ≤ 0.05) determined by Kruskal-Wallis test.