Graphical representation of BLASTn comparison of integrative conjugative elements of PA34.

Protein-coding regions are represented by the orange arrows and key features/associated genes are shown. The gradient grey shading represents regions of nucleotide sequence identity (100% to 65%) determined by BLASTn analysis. Figures are drawn to scale using Easyfig [35]. (A) A comparison of two genomic islands (RGP 29 and RGP5) of PA34 with a clc-like integrative conjugative element (Genbank Accession number AJ617740) (parA = plasmid partition protein, traD = conjugative factor, intB13 = phage integrase, merA/B/R = mercury resistance operon, clc = chlorocatechol (clc) degradation). (B) A comparison of the genomic island RGP41 of PA34 with pKLC102 (GenBank Accession number AY257538) and PAPI1(GenBank Accession number AY273869) (soj = chromosome partitioning protein, dnaB = replicative DNA helicase, ssb = single strand binding protein, pilP/pilV = type IV pilus biogenesis and transfer, traD = conjugative factor, cupD/rcsC/pvrR = cell surface fimbriae operon, parE = plasmid stabilisation protein, xerC = integrase).