Quantitative real-time PCR analysis of <i>PTGDR</i> gene expression after ATRA or DMSO treatment in KU812 cells.
- Asunción García-Sánchez (4557109)
- Elena Marcos-Vadillo (4557103)
- Catalina Sanz (346521)
- Miguel Estravís (6589322)
- María Isidoro-García (4557100)
- Ignacio Dávila (6589325)
Publication date
April 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
A. KU812 cells were treated with 1 μM ATRA or DMSO and collected after 24 and 48h followed by qPCR analysis as above mentioned. Data are shown as fold increase compared with DMSO (calibrator). * PB. KU812 cells were pre-treated with 1 μM of Pan RAR-antagonist (AGN 193109) or RARβ antagonist (CD 2665) 1 hour before induction with ATRA or DMSO for 24 and 48 h. Cells treated with ATRA or DMSO were used as control. Data are shown as Log10 fold increase compared with DMSO treated cells at the same conditions (calibrator). ** P<0.01 for ATRA 48h no antagonist compared with ATRA 48h RARβ antagonist (Student´s T-test).</p
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