Morphology of Der2^SCKO and S63del//Der2^SCKO adult sciatic nerves.

(A) Transverse semithin sections from WT, Der2SCKO, S63del and S63del//Der2SCKO sciatic nerves at 12 mo. Arrowheads indicate demyelinating/remyelinating fibers. n = 4–5 mice/genotype. Scale bar, 10μm; 100x magnification. (B) Morphometric g-ratio analysis performed on sciatic nerve semithin sections at 6 mo. Scatter-plot of g-ratio distribution with trend lines is reported; ~800–1200 fibers/genotype. n = 3–4 nerves per genotype. (C) Number of demyelinated fibers in 6 and 12 mo sciatic nerves (expressed as a percentage of total myelinated fibers per sciatic nerve field). 8–10 non-overlapping fields per nerve were analyzed from n = 3–5 nerves per genotype. Error bars, SEM; * and § P t test. (D) Transverse semithin sections of 6 mo Der2SCKO and S63del//Der2SCKO motor fascicles of sciatic nerves in which large onion bulbs and naked axons are visible (arrowheads). Scale bar, 10μm.