Selective colony area method for heterogeneous patient-derived tumor cell lines in anti-cancer drug screening system - Fig 2

(a) Cell images and area reducing ratio of KATO III human gastric cancer cell line and #2 patient-derived cancer cell line (PDC) according to intensity thresholds. Red mark in the images are the selected colonies according to intensity thresholds. (b) Colony images and IC50 values comparison between MKN1 human gastric cancer cell line and #1 PDC. Contrary to MKN1 gastric cancer cell line image, colony formation was seen only with some of the cells from the #1 PDC. In the five PDCs, IC50 values calculated by the selected area were generally lower than those calculated using total intensity. Ten outliers (red circles) who IC50 difference between total intensity and selected area analysis is more than 1 dose (3 times) were observed.