Effect of 10-undecynoic acid on the biofilm growth of <i>Streptococcus mutans</i> UA159 and <i>Streptococcus sobrinus</i> SL1.

(A-C) MBIC values were determined on 96-well plates coated with human saliva after 24 h incubation with 10-undecynoic acid and assessed by alamarBlue and crystal violet. Inserts: representative images of biofilms of Streptococcus sp. formed on artificial teeth coated with human saliva after 24 h incubation period with 10-undecynoic acid. (D) Representative images of biofilms of Streptococcus mutans UA159 and Streptococcus sobrinus SL1 stained with SYTO9 after 24 h incubation period with 5.0 mg/ml of 10-undecynoic acid. Control– 0.1% DMSO, 10-UDYA– 10-undecynoic acid, * p<0.001 compared to control, RFU–relative fluorescence unit.