Fluid supplementation ameliorates signs of colitis without altering mouse behavior.

A) Diagram showing the procedures that mice underwent throughout the whole study. One mL of 0.9% NaCl solution was injected IP in fluid-supplemented mice from days 3 to 6 after beginning of DSS administration. B) Body weight variation (n = 16–17 until day 7, n = 9 from days 7–12; *PPPC) DAI (n = 16–17 until day 7, n = 9 from days 7–12; *PPPPD) Tail suspension test (TST; n = 11–12; two-tailed t-test). E) Novel object recognition test (NOR; n = 11–12; two-tailed T-test comparing interactions with novel object). F) Open field activity test (n = 11–12; two-way ANOVA). G) Rearing behavior (n = 11–12; two-way ANOVA).