Freezing tendons in DMSO retain scleraxis positive cells, which can be put in cell culture.
- Eva Hochstrat (6616373)
- Marcus Müller (68469)
- Andre Frank (6616376)
- Philipp Michel (6616379)
- Uwe Hansen (108379)
- Michael J. Raschke (6616382)
- Daniel Kronenberg (6616385)
- Richard Stange (6616388)
Publication date
April 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
After 7 days of cultivation, tendon-derived cells can be detected either in freshly cultivated as well as in in DMSO frozen tendons (A). Quantification of MTT -assay of tendon derived cells after 8 days of cultivation (B). Quantitative real-time PCR of the resident cells from freshly prepared, in PBS and DMSO-frozen tendons, testing the scleraxis expression(C).</p
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