Interaction of GhMFT1 and GhMFT2 with GhFD <i>in vivo</i>.

(A) Yeast two-hybrid assay of protein interaction. Transformed yeast cells harboring GhFD fused to activation domain (AD), and GhMFT1, GhMFT2 fused to binding domain (BD) were grown on selection media without Leu, Trp, His and Ade (-LTHA) indicating positive interactions. (B) Bimolecular fluorescence complementation analysis of protein interactions both GhMFT1 and GhMFT2 with GhFD in Arabidopsis protoplast cells. YFP, YFP fluorescence; Chlorophyll represents chloroplast auto fluorescence; Bright, bright field image; Merge represents merge of the former three images. Scale bar, 5 μm.