Supplemental Figure 2

Figure S2. 8-br-cAMP increases PA103-induced caspase-1 activation in PMVECs in a dose-dependent manner. PMVECs were serum starved for 60 min. The 8-br-cAMP (panel A) and 8-br-cGMP (panel B) compounds were added to the culture medium 30 min prior to addition of FLICA reagent and PA103 (MOI 10:1). Saline-treated PMVEC monolayers served as negative controls. PMVECs were harvested at 180 min post-infection and analyzed for activated caspase-1 by flow cytometry. For all conditions n=3. For 8-br-cAMP in panel A, the (*) indicates a significant difference, P