Additional file 5: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Additional file 1: of Nucleus- and plastid-targeted annexin 5 promotes reproductive development in Arabidopsis and is essential for pollen and embryo formation

Malgorzata Lichocka (769738)
Wojciech Rymaszewski (769735)
Karolina Morgiewicz (5495822)
Izabela Barymow-Filoniuk (5495825)
Aleksander Chlebowski (5029667)
Miroslaw Sobczak (147854)
Marcus Samuel (5726018)
Elmon Schmelzer (174039)
Magdalena Krzymowska (5423816)
Jacek Hennig (769746)

September 2018

Table S1. List of binary plasmids used in this study. Table S2. Oligonucleotides used for RT-qPCR. F...

Additional file 1: of A comparison of heat-stress transcriptome changes between wild-type Arabidopsis pollen and a heat-sensitive mutant harboring a knockout of cyclic nucleotide-gated cation channel 16 (cngc16)

Maryam Rahmati Ishka (5555981)
Elizabeth Brown (281332)
Chrystle Weigand (5555984)
Richard Tillett (3874717)
Karen Schlauch (3402116)
Gad Miller (680362)
Jeffrey Harper (5555978)

July 2018

Hot/Cold stress cycle. Diagram showing the Hot/Cold stress-cycle used here for growing plants from w...

Additional file 3: of Proline synthesis in developing microspores is required for pollen development and fertility

Roberto Mattioli (3491525)
Marco Biancucci (459739)
Amira Shall (6093233)
Luciana Mosca (83281)
Paolo Costantino (1882918)
Dietmar Funck (6093236)
Maurizio Trovato (3491534)

December 2018

Figure S3. Histochemical localization of GUS activity in anthers of p35S:GUS, pLtp12:GUS and p17340:...

Additional file 4: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Ferdousse Laggoun (6620645)
Flavien Dardelle (6620648)
Jérémy Dehors (6599468)
Denis Falconet (6024)
Azeddine Driouich (331954)
Christophe Rochais (1621765)
Patrick Dallemagne (1621738)
Arnaud Lehner (6599474)
Jean-Claude Mollet (6599477)

April 2019

Video S2. Time-lapse imaging of actin dynamics in Arabidopsis thaliana pollen tubes treated for 6 h ...

Additional file 3: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Ferdousse Laggoun (6620645)
Flavien Dardelle (6620648)
Jérémy Dehors (6599468)
Denis Falconet (6024)
Azeddine Driouich (331954)
Christophe Rochais (1621765)
Patrick Dallemagne (1621738)
Arnaud Lehner (6599474)
Jean-Claude Mollet (6599477)

April 2019

Video S1. Time-lapse imaging of actin dynamics in untreated Arabidopsis thaliana pollen tubes after ...

Additional file 7: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Ferdousse Laggoun (6620645)
Flavien Dardelle (6620648)
Jérémy Dehors (6599468)
Denis Falconet (6024)
Azeddine Driouich (331954)
Christophe Rochais (1621765)
Patrick Dallemagne (1621738)
Arnaud Lehner (6599474)
Jean-Claude Mollet (6599477)

April 2019

Figure S4. Time-lapse imaging of RIC4 dynamics of a pollen tube treated with Disruptol-B for 2 h. (P...

Additional file 6: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Ferdousse Laggoun (6620645)
Flavien Dardelle (6620648)
Jérémy Dehors (6599468)
Denis Falconet (6024)
Azeddine Driouich (331954)
Christophe Rochais (1621765)
Patrick Dallemagne (1621738)
Arnaud Lehner (6599474)
Jean-Claude Mollet (6599477)

April 2019

Figure S3. Time-lapse imaging of RIC4 dynamics in Arabidopsis thaliana pollen tubes treated for 2, 4...

Additional file 1: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Ferdousse Laggoun (6620645)
Flavien Dardelle (6620648)
Jérémy Dehors (6599468)
Denis Falconet (6024)
Azeddine Driouich (331954)
Christophe Rochais (1621765)
Patrick Dallemagne (1621738)
Arnaud Lehner (6599474)
Jean-Claude Mollet (6599477)

April 2019

Figure S1. Dose-response effect of the compounds on (a) Solanum lycopersicum and (b) Nicotiana tabac...

A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Laggoun, Ferdousse
Dardelle, Flavien
Dehors, Jérémy
Falconet, Denis
Driouich, Azeddine
Rochais, Christophe
Dallemagne, Patrick
Lehner, Arnaud
Mollet, Jean-Claude

January 2019

International audienceBackground: During sexual reproduction, pollen grains land on the stigma, rehy...

Additional file 3: of Efficient preparation of Arabidopsis pollen tubes for ultrastructural analysis using chemical and cryo-fixation

Tohnyui Ndinyanka Fabrice (4541890)
Andres Kaech (225163)
Gery Barmettler (4541896)
Christof Eichenberger (4541893)
J. Paul Knox (491206)
Ueli Grossniklaus (3152)
Christoph Ringli (512544)

October 2017

Comparison of the ChF, PF, and HPF ultrastructural details of PTs within the pistil. The intracellul...

Additional file 1: of Efficient preparation of Arabidopsis pollen tubes for ultrastructural analysis using chemical and cryo-fixation

Tohnyui Ndinyanka Fabrice (4541890)
Andres Kaech (225163)
Gery Barmettler (4541896)
Christof Eichenberger (4541893)
J. Paul Knox (491206)
Ueli Grossniklaus (3152)
Christoph Ringli (512544)

October 2017

Additional Methods. Detailed step-by-step procedure and list of material needed for growth of PTs an...

Apical actin polymerization is impaired in formin loss-of-function mutant pollen tubes.

Yaxian Lan (5954108)
Xiaonan Liu (142071)
Ying Fu (173963)
Shanjin Huang (35466)

November 2018

(A) Time-lapse images of actin filaments in growing WT and fh3-1, fh5-2 and fh3-1 fh5-2 mutant polle...

Additional file 2: Figure S2. of Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization

Said Hafidh (265846)
David Potěšil (3426254)
Jan Fíla (3426260)
Věra Čapková (3426257)
Zbyněk Zdráhal (3324678)
David Honys (57731)

May 2016

Assessment of pollen tube integrity, secretome purity using an alcohol-dehydrogenase (ADH) assay, an...

Additional file 3: of A 3-dimensional fibre scaffold as an investigative tool for studying the morphogenesis of isolated plant cells

CJ Luo (3430487)
Raymond Wightman (3430493)
Elliot Meyerowitz (3430490)
Stoyan K. Smoukov (1275561)

August 2015

A Windows Media movie Movie S2.avi showing a 824 4D dataset of microtubule dynamics. White arrow sho...

Additional file 1: of Proline synthesis in developing microspores is required for pollen development and fertility

Roberto Mattioli (3491525)
Marco Biancucci (459739)
Amira Shall (6093233)
Luciana Mosca (83281)
Paolo Costantino (1882918)
Dietmar Funck (6093236)
Maurizio Trovato (3491534)

December 2018

Figure S1. Histochemical localization of GUS activity in anthers of pP5CS1:GUS and pP5CS2:GUS transg...

Additional file 1: of Nucleus- and plastid-targeted annexin 5 promotes reproductive development in Arabidopsis and is essential for pollen and embryo formation

Malgorzata Lichocka (769738)
Wojciech Rymaszewski (769735)
Karolina Morgiewicz (5495822)
Izabela Barymow-Filoniuk (5495825)
Aleksander Chlebowski (5029667)
Miroslaw Sobczak (147854)
Marcus Samuel (5726018)
Elmon Schmelzer (174039)
Magdalena Krzymowska (5423816)
Jacek Hennig (769746)

September 2018

Table S1. List of binary plasmids used in this study. Table S2. Oligonucleotides used for RT-qPCR. F...

Additional file 1: of A comparison of heat-stress transcriptome changes between wild-type Arabidopsis pollen and a heat-sensitive mutant harboring a knockout of cyclic nucleotide-gated cation channel 16 (cngc16)

Maryam Rahmati Ishka (5555981)
Elizabeth Brown (281332)
Chrystle Weigand (5555984)
Richard Tillett (3874717)
Karen Schlauch (3402116)
Gad Miller (680362)
Jeffrey Harper (5555978)

July 2018

Hot/Cold stress cycle. Diagram showing the Hot/Cold stress-cycle used here for growing plants from w...

Additional file 3: of Proline synthesis in developing microspores is required for pollen development and fertility

Roberto Mattioli (3491525)
Marco Biancucci (459739)
Amira Shall (6093233)
Luciana Mosca (83281)
Paolo Costantino (1882918)
Dietmar Funck (6093236)
Maurizio Trovato (3491534)

December 2018

Figure S3. Histochemical localization of GUS activity in anthers of p35S:GUS, pLtp12:GUS and p17340:...

Additional file 4: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Ferdousse Laggoun (6620645)
Flavien Dardelle (6620648)
Jérémy Dehors (6599468)
Denis Falconet (6024)
Azeddine Driouich (331954)
Christophe Rochais (1621765)
Patrick Dallemagne (1621738)
Arnaud Lehner (6599474)
Jean-Claude Mollet (6599477)

April 2019

Video S2. Time-lapse imaging of actin dynamics in Arabidopsis thaliana pollen tubes treated for 6 h ...

Additional file 3: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Ferdousse Laggoun (6620645)
Flavien Dardelle (6620648)
Jérémy Dehors (6599468)
Denis Falconet (6024)
Azeddine Driouich (331954)
Christophe Rochais (1621765)
Patrick Dallemagne (1621738)
Arnaud Lehner (6599474)
Jean-Claude Mollet (6599477)

April 2019

Video S1. Time-lapse imaging of actin dynamics in untreated Arabidopsis thaliana pollen tubes after ...

Additional file 7: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Ferdousse Laggoun (6620645)
Flavien Dardelle (6620648)
Jérémy Dehors (6599468)
Denis Falconet (6024)
Azeddine Driouich (331954)
Christophe Rochais (1621765)
Patrick Dallemagne (1621738)
Arnaud Lehner (6599474)
Jean-Claude Mollet (6599477)

April 2019

Figure S4. Time-lapse imaging of RIC4 dynamics of a pollen tube treated with Disruptol-B for 2 h. (P...

Additional file 6: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Ferdousse Laggoun (6620645)
Flavien Dardelle (6620648)
Jérémy Dehors (6599468)
Denis Falconet (6024)
Azeddine Driouich (331954)
Christophe Rochais (1621765)
Patrick Dallemagne (1621738)
Arnaud Lehner (6599474)
Jean-Claude Mollet (6599477)

April 2019

Figure S3. Time-lapse imaging of RIC4 dynamics in Arabidopsis thaliana pollen tubes treated for 2, 4...

Additional file 1: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Ferdousse Laggoun (6620645)
Flavien Dardelle (6620648)
Jérémy Dehors (6599468)
Denis Falconet (6024)
Azeddine Driouich (331954)
Christophe Rochais (1621765)
Patrick Dallemagne (1621738)
Arnaud Lehner (6599474)
Jean-Claude Mollet (6599477)

April 2019

Figure S1. Dose-response effect of the compounds on (a) Solanum lycopersicum and (b) Nicotiana tabac...

A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Laggoun, Ferdousse
Dardelle, Flavien
Dehors, Jérémy
Falconet, Denis
Driouich, Azeddine
Rochais, Christophe
Dallemagne, Patrick
Lehner, Arnaud
Mollet, Jean-Claude

January 2019

International audienceBackground: During sexual reproduction, pollen grains land on the stigma, rehy...

Additional file 3: of Efficient preparation of Arabidopsis pollen tubes for ultrastructural analysis using chemical and cryo-fixation

Tohnyui Ndinyanka Fabrice (4541890)
Andres Kaech (225163)
Gery Barmettler (4541896)
Christof Eichenberger (4541893)
J. Paul Knox (491206)
Ueli Grossniklaus (3152)
Christoph Ringli (512544)

October 2017

Comparison of the ChF, PF, and HPF ultrastructural details of PTs within the pistil. The intracellul...

Additional file 1: of Efficient preparation of Arabidopsis pollen tubes for ultrastructural analysis using chemical and cryo-fixation

Tohnyui Ndinyanka Fabrice (4541890)
Andres Kaech (225163)
Gery Barmettler (4541896)
Christof Eichenberger (4541893)
J. Paul Knox (491206)
Ueli Grossniklaus (3152)
Christoph Ringli (512544)

October 2017

Additional Methods. Detailed step-by-step procedure and list of material needed for growth of PTs an...

Apical actin polymerization is impaired in formin loss-of-function mutant pollen tubes.

Yaxian Lan (5954108)
Xiaonan Liu (142071)
Ying Fu (173963)
Shanjin Huang (35466)

November 2018

(A) Time-lapse images of actin filaments in growing WT and fh3-1, fh5-2 and fh3-1 fh5-2 mutant polle...

Additional file 2: Figure S2. of Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization

Said Hafidh (265846)
David Potěšil (3426254)
Jan Fíla (3426260)
Věra Čapková (3426257)
Zbyněk Zdráhal (3324678)
David Honys (57731)

May 2016

Assessment of pollen tube integrity, secretome purity using an alcohol-dehydrogenase (ADH) assay, an...

Additional file 3: of A 3-dimensional fibre scaffold as an investigative tool for studying the morphogenesis of isolated plant cells

CJ Luo (3430487)
Raymond Wightman (3430493)
Elliot Meyerowitz (3430490)
Stoyan K. Smoukov (1275561)

August 2015

A Windows Media movie Movie S2.avi showing a 824 4D dataset of microtubule dynamics. White arrow sho...

Additional file 1: of Proline synthesis in developing microspores is required for pollen development and fertility

Roberto Mattioli (3491525)
Marco Biancucci (459739)
Amira Shall (6093233)
Luciana Mosca (83281)
Paolo Costantino (1882918)
Dietmar Funck (6093236)
Maurizio Trovato (3491534)

December 2018

Figure S1. Histochemical localization of GUS activity in anthers of pP5CS1:GUS and pP5CS2:GUS transg...

Additional file 1: of Nucleus- and plastid-targeted annexin 5 promotes reproductive development in Arabidopsis and is essential for pollen and embryo formation

Malgorzata Lichocka (769738)
Wojciech Rymaszewski (769735)
Karolina Morgiewicz (5495822)
Izabela Barymow-Filoniuk (5495825)
Aleksander Chlebowski (5029667)
Miroslaw Sobczak (147854)
Marcus Samuel (5726018)
Elmon Schmelzer (174039)
Magdalena Krzymowska (5423816)
Jacek Hennig (769746)

September 2018

Table S1. List of binary plasmids used in this study. Table S2. Oligonucleotides used for RT-qPCR. F...

Additional file 1: of A comparison of heat-stress transcriptome changes between wild-type Arabidopsis pollen and a heat-sensitive mutant harboring a knockout of cyclic nucleotide-gated cation channel 16 (cngc16)

Maryam Rahmati Ishka (5555981)
Elizabeth Brown (281332)
Chrystle Weigand (5555984)
Richard Tillett (3874717)
Karen Schlauch (3402116)
Gad Miller (680362)
Jeffrey Harper (5555978)

July 2018

Hot/Cold stress cycle. Diagram showing the Hot/Cold stress-cycle used here for growing plants from w...

Additional file 3: of Proline synthesis in developing microspores is required for pollen development and fertility

Roberto Mattioli (3491525)
Marco Biancucci (459739)
Amira Shall (6093233)
Luciana Mosca (83281)
Paolo Costantino (1882918)
Dietmar Funck (6093236)
Maurizio Trovato (3491534)

December 2018

Figure S3. Histochemical localization of GUS activity in anthers of p35S:GUS, pLtp12:GUS and p17340:...

Additional file 5: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Abstract

Extracted data

Additional file 5: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Abstract

Extracted data

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