Differential levels of nuclear import factors in the two halves of an early embryo lead to bent tadpoles.

(A) Two-cell embryos were microinjected as indicated and allowed to develop into 9 dpf swimming tadpoles. Representative images are shown. Single-headed arrows indicate small eyes. Double-headed arrows indicate bent bodies. (B) Tadpoles were scored as indicated by measuring eye areas and body axis angles. Embryo numbers: n = 10 for GFP, n = 25 for NTF2, n = 18 for imp α + GFP-LB3/NTF2.