Viremia and RNAemia detected after challenge of Gr.3 and 5 with either DENV-2 0126/2010 or DENV-2 S16803.

At month 8.5 post-second immunization, Gr.3 and 5 were divided into two subgroups each (n = 5 but Gr.3/DENV-2 S16803, n = 4) which were subcutaneously inoculated with approximately 4x104 plaque-forming units (PFU) of DENV-2 S16803 or approximately 105 PFU of DENV-2 0126/2010. (A) Shown are the individual viremia (expressed as plaque-forming units (PFU)/mL) and RNAemia (expressed as genome equivalent (ge)/mL) curves determined using either fresh (solid symbols) or frozen-thawed (open symbols) sera. Horizontal black and grey dotted lines indicate the threshold of detection for viremia and RNAemia, respectively. Horizontal green and red dashed lines indicate the lowest and highest RNAemia peaks detected in the corresponding non-vaccinated subgroup. Animals with RNAemia peaks higher than the highest peaks detected in non-vaccinated groups are indicated in red font. (B) Shown are the geometric mean ratio (GMR) and 95% confidence intervals (CI) for viremia and RNAemia area under the curves (AUC) determined using fresh versus frozen-thawed sera. Statistical comparisons were based on a paired t-test (*, pp<0.05).