Neutrophil analysis in co-housed wild-type and <i>Stat2^-/-</i> mice infected with <i>S.</i> Typhimurium.

A. for the cecum of uninfected and S. Typhimurium infected wild-type C57BL/6 and Stat2-/- mice. B. Average neutrophils (Cumulative histopathology score PMN) numbers enumerated in ten fields from cecum of uninfected and S. Typhimurium infected wild-type C57BL/6 and Stat2-/- mice. C. Flow cytometry analysis of cecal cell suspensions. Neutrophils were gated after duplet and dead cell elimination for the following markers; CD45+, CD3-, CD56-, CD19-, Ly6G+ D. Percent of live cells as determined by flow cytometry for C57BL/6 uninfected, C57BL/6 S. Typhimurium infected and Stat2-/- S. Typhimurium infected mice. E. Superoxide anion (O2-) generation in response to fMLP in the presence or absence of IFNα by bone marrow neutrophils isolated from wild-type and Stat2-/- mice. Mean and SE were calculated by averaging results from three independent experiments. *p <0.05, ** p<0.01 as determined by Students t-test.