Additional file 2: of Advanced assessment of migration and invasion of cancer cells in response to mifepristone therapy using double fluorescence cytochemical labeling

Figure S2. Enhancing the wound healing assay (2D migration assay) and the Boyden chamber assay (3D migration assay) with a double fluorescence labelling allows for the visualization of the position of the nucleus relative to the cytoplasm in migrating cells. U87MG (A) and SKOV-3 (B) were subjected to migration in a wound healing assay. MDA-MB-231 (C) and LNCaP (D) were subjected to migration in a Boyden chamber assay. Arrows, nucleus at the back of the cell; arrowheads, nuclei at the front of the cell. White lines in A and B mark the border of the wound. Scale bars = 75 μm. (TIF 6039 kb