A and B. LdAAP3 and LdPT levels of intracellular amastigotes treated with external arginine. THP-1 cells were infected with L. donovani in RPMI medium containing 0.1 mM arginine for 48 h. They were then treated with 1 mM or 5 mM of arginine for 2 h. The total RNA was extracted, and the resulting cDNA was subjected to real-time PCR analysis using primers specific for LdAAP3 (A) and LdPT (B). The results are expressed as fold-change of control (2 h infected and untreated cells). Values are mean ± S.E.M. (n = 3). The results are representative of three independent experiments performed in triplicates.</p
<p> <b>A</b>) Arginase activity in macrophages isolated by adherence from single-cell suspensions fr...
<p>(A) Groups of BALB/c and CBA mice (n = 4) were infected with <i>L. major</i> parasites in one hin...
<p>Macrophages (2×10<sup>6</sup> cells) were infected with <i>L. donovani</i> (1∶10) and incubated a...
A–C. Real-time PCR to monitor LdAAP3 and LdPT levels in intracellular amastigotes treated with argin...
A. The minimal arginine concentration required for activation of ADR in axenic promastigotes. The pr...
<p><i>Leishmania</i> parasites were grown in L-arginine depleted (AD-<i>Ld</i>), L-arginine depleted...
<p>A) Time-course of LdAAP3 protein abundance increase as function of arginine deprivation time in w...
A and B. The effect of arginine transport inhibitors on ADR was checked. Axenic promastigotes were c...
A. The effect of three different arginine transport inhibitors was checked on ADR. Axenic promastigo...
<p>LdAAP3.2 mRNA (LinJ.31.0910) abundance in axenic <i>L</i>. <i>donovani</i> promastigotes and amas...
<p>(A-B) <i>Leishmania</i> parasites were grown in L-arginine depleted (AD-<i>Ld</i>) and L-arginine...
In the field of infectious diseases the multifaceted amino acid arginine has reached special attenti...
<p>Arginine deprivation-induced protein phosphorylation in <i>L</i>. <i>donovani</i> promastigotes.<...
In the field of infectious diseases the multifaceted amino acid arginine has reached special attenti...
<p>WT, OE and <i>LdASS</i><sup>-/-/+</sup> parasites were cultured in L-arginine free RPMI-1640, L-a...
<p> <b>A</b>) Arginase activity in macrophages isolated by adherence from single-cell suspensions fr...
<p>(A) Groups of BALB/c and CBA mice (n = 4) were infected with <i>L. major</i> parasites in one hin...
<p>Macrophages (2×10<sup>6</sup> cells) were infected with <i>L. donovani</i> (1∶10) and incubated a...
A–C. Real-time PCR to monitor LdAAP3 and LdPT levels in intracellular amastigotes treated with argin...
A. The minimal arginine concentration required for activation of ADR in axenic promastigotes. The pr...
<p><i>Leishmania</i> parasites were grown in L-arginine depleted (AD-<i>Ld</i>), L-arginine depleted...
<p>A) Time-course of LdAAP3 protein abundance increase as function of arginine deprivation time in w...
A and B. The effect of arginine transport inhibitors on ADR was checked. Axenic promastigotes were c...
A. The effect of three different arginine transport inhibitors was checked on ADR. Axenic promastigo...
<p>LdAAP3.2 mRNA (LinJ.31.0910) abundance in axenic <i>L</i>. <i>donovani</i> promastigotes and amas...
<p>(A-B) <i>Leishmania</i> parasites were grown in L-arginine depleted (AD-<i>Ld</i>) and L-arginine...
In the field of infectious diseases the multifaceted amino acid arginine has reached special attenti...
<p>Arginine deprivation-induced protein phosphorylation in <i>L</i>. <i>donovani</i> promastigotes.<...
In the field of infectious diseases the multifaceted amino acid arginine has reached special attenti...
<p>WT, OE and <i>LdASS</i><sup>-/-/+</sup> parasites were cultured in L-arginine free RPMI-1640, L-a...
<p> <b>A</b>) Arginase activity in macrophages isolated by adherence from single-cell suspensions fr...
<p>(A) Groups of BALB/c and CBA mice (n = 4) were infected with <i>L. major</i> parasites in one hin...
<p>Macrophages (2×10<sup>6</sup> cells) were infected with <i>L. donovani</i> (1∶10) and incubated a...