Stat1^-/- mice display increased production and proteolytic maturation of IL-1β upon gastrointestinal MNV infection.

Age- and sex-matched Stat1+/- and Stat1-/- littermates were infected with 10/ PFU of UV-inactivated (UV) or live MNV via oral gavage. At 3 days post-infection ileum, Peyer`s patches (PP), mesenteric lymph nodes (MLN), spleen and liver lysates were prepared for Multiplex ELISA (A, B) and Western Blotting analyses (C-E). (A) Heat maps showing relative expression of chemokines and cytokines as measured by Multiplex ELISA in the indicated tissues of UV- or Live MNV infected Stat1+/- and Stat1-/-mice. Data shown are the means of Stat1+/-UV-MNV mice (n = 7), Stat1+/-Live-MNV mice (n = 8), Stat1-/-UV-MNV mice (n = 5) and Stat1-/-Live-MNV mice (n = 8), normalized per mean log2 expression. (B) Raw data from heat map analysis showing IL-1β levels in indicated tissues. Samples indicated in blue and red were analyzed by (C-E) Western blotting for proteolytic maturation of IL-1β in (C) MLN, (D) spleen and (E) liver. Statistics: Log-linear regression analysis, p values in (A, B) indicate association with Stat1-/-Live-MNV set-up, with *p<0.05; **p<0.01; ***p<0.001.