Additional file 2: of Fn14 overcomes cisplatin resistance of high-grade serous ovarian cancer by promoting Mdm2-mediated p53-R248Q ubiquitination and degradation

Figure S2. Characterization of primary HGSOC cells and identification of p53 status in primary cells. (A) Primary cells were photographed by light micrographs and CK7 (green) and Vimentin (red) expression was determined by Immunofluorescence. PI (blue) staining shows the nuclei, magnification of 200×. (B) and (C) The sequence of R248Q p53-coding region in primary cells. (D) Expression of Fn14 in four HGSOC cells was determined by western blot. (E) CCK-8 assay was performed to detect IC50 of CDDP in HGSOC cells. (TIF 2666 kb