Additional file 6: of Mammalian Eps15 homology domain 1 potentiates angiogenesis of non-small cell lung cancer by regulating β2AR signaling

Figure S2. Reexpression of EHD1 promotes angiogenesis. a Western blot analysis of EHD1 expression in A549 and NCI-H1650 cells after EHD1 reexpression. b The viability of HUVECs was detected by CCK8 assay. HUVECs were incubated in 96-well plates with CMs from A549 and NCI-H1650 cells. c CMs were added to the lower chamber, and HUVECs were seeded on the upper chamber. After 24 h of incubation, HUVEC migration was assessed by counting the cells on the lower surface of the membrane; from left to right, the lanes show Sh/UT, Sh/Ctrl and Sh/R. Scale bar, 100 μm. d HUVECs were incubated in 48-well plates with CMs from A549 and NCI-H1650 cells, and their tube formation abilities were evaluated based on the number of tubes per field. *p < 0.05; **p < 0.01. (TIF 10271 kb