ITK knock-down impairs Th17 and promotes Th1 differentiation.

Human naïve CD4 T cells were treated with NTC or sd-ITK and then activated under Th17 or Th1 polarizing conditions. Four days later culture supernatants were assessed for IL-17A by Luminex (A; N = 5), and CD4+ T cells were analyzed for CCR6 (B; N = 3) or INFɣ (C; N = 3) by flow cytometry. Right panels show representative flow cytometry plots of T cells treated with NTC or sd-ITK. Median ± IQR, *p<0.05 (paired two-tailed Student’s t-test).