ITK knock-down induces bona-fide suppressive T_REG.

Human naïve T cells treated with NTC or sd-ITK and differentiated under TREG polarizing conditions (TREG-polarized) were labeled with CVT, and CD4+CD25Neg responder T cells were labeled with CFSE (T-responder). TREG and T-responder were cultured in the presence of anti-CD3/CD28 activation beads. Flow cytometry analysis was performed after 4 days. (A) Division index for each TREG-polarized/T-responder ratio was calculated as the average number of cell divisions of the original CFSE-labeled population versus the undivided CFSEhigh cells using the FlowJo proliferation platform. (B) Representative flow cytometry plots using a 1:1 TREG-polarized/T-responder ratio. N = 4 donors. Median ± IQR, *pMethods).