Additional file 1: of Age-related dysfunction of the DNA damage response in intestinal stem cells

Figure S1. Expression patterns of Ki-67. Proliferating progenitor cells were stained with an antibody against Ki-67 in the transit amplifying compartment. Scale bar: 50 Îźm. Figure S2. Immunohistochemical analyses of cleaved Caspase-3. GFP and cleaved Caspase-3 double-positive apoptotic intestinal stem cells (ISCs) were observed in irradiated young crypts. Arrows and arrowheads indicate GFP and cleaved Caspase-3 double-positive and GFP single-positive cells, respectively. Scale bar: 50 Îźm. Figure S3. IL-6-positive Paneth cells were observed in old crypts. Expression of IL-6 was observed in old lysozyme-positive Paneth cells in old crypts before irradiation. Arrows and an arrowhead indicate lysozyme and IL-6 double-positive and lysozyme single-positive cells, respectively. Scale bar: 50 Îźm. Table S1. List of mice. (PDF 743 kb