Liver cell infectivity of rH17d-containing Ad5 vectors.

(A) Baseline infectivity of the Ad5 vectors was established by incubating TIB76 liver cells with rH17d’- or His6-modified adenoviruses for 1 h in PBS (5 x108 VP). Infectivity levels were determined by quantification of luciferase transgene expression in infected cells 24h post-transduction. (B) The Ad5 vectors were also pre-incubated with serum from normal C57BL/6 mice and then used to infect TIB76 liver cells. Luciferase transgene expression in virus-infected cells was quantified and normalized to the respective infectivity levels observed in PBS (without pre-incubation with serum). All data represent the mean ± SEM from 3 independent experiments and 4 replicates (n = 4) each; *p6 control.