MOESM7 of Interaction of HIV-1 integrase with polypyrimidine tract binding protein and associated splicing factor (PSF) and its impact on HIV-1 replication

Additional file 7: Figure S7. Quantitative PCR of HIV-1 2-LTR, integrated provirus and cDNA per cell—The TZM-bl cell line was overexpressed with the GFP-PSF plasmid and GFP was used as a control plasmid. Cells were transduced with the pNL4-3 virus at 0.5 MOI and DNA was detected by qPCR at different time points. [A] 2-LTR detection at different time point after overexpression of cell, infecting with pNL4-3 virus and harvesting DNA for analysis. [B] Integrated provirus was detected by Alu nested PCR at 24 and 48 h after infection with pNL4-3 at 0.5 MOI and extracting DNA from overexpressed TZM-bl cells. Paired t test analysis revealed p values < 0.05. Error bar depicts SD between three independent experiment. [C] cDNA detection at different time point after overexpression of PSF inside the cell