Impaired PI metabolism in patient-derived fibroblasts.
- Dov Tiosano (6645557)
- Hagit N. Baris (5472464)
- Anlu Chen (4479259)
- Marrit M. Hitzert (6645560)
- Markus Schueler (229810)
- Federico Gulluni (6645563)
- Antje Wiesener (390053)
- Antonio Bergua (6645566)
- Adi Mory (6645569)
- Brett Copeland (4631320)
- Joseph G. Gleeson (779314)
- Patrick Rump (3536780)
- Hester van Meer (6645572)
- Deborah A. Sival (6645575)
- Volker Haucke (1859740)
- Josh Kriwinsky (6645578)
- Karl X. Knaup (186120)
- André Reis (166910)
- Nadine N. Hauer (6645581)
- Emilio Hirsch (113836)
- Ronald Roepman (480772)
- Rolph Pfundt (249799)
- Christian T. Thiel (390057)
- Michael S. Wiesener (186173)
- Mariam G. Aslanyan (6645584)
- David A. Buchner (530142)
DOIAbstract
Quantification of PI(3,4)P2 and PI(3)P were analysed and normalized on whole cell fluorescence. (A) Immunofluorescence analysis of cellular PI(3,4)P2 levels. Results showed that PI(3,4)P2 is significantly reduced throughout the cell in -/- cells compared with +/+ cells. n = 4 independent experiments and 10 cells per genotype for each experiment (S6 Table). (B) Immunofluorescence analysis of PI(3)P localization at the base of the primary cilium. Results showed that PI(3)P is significantly reduced at the base of the primary cilium in -/- cells compared with +/+ and or +/- cells. n = 3 independent experiments and 15 cells per genotype for each experiment (S6 Table). Nuclei are stained with DAPI. ** indicates p < 0.01. *** indicates p < 0.0001....
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