Early life exposure to high temperature promotes persistent stress responses.

(A) Schematic of the analysis of stress responses after temperature shift. (B) Worms were cultivated at 25°C until day 1 adults and transferred to 15°C thereafter. T24B8.5p::GFP expression was examined as indicated in 3A. (C) Quantification of GFP levels. Number of biological replicates (n): 15°C day 1 (98), 25°C day 1 (124), 15°C day 4 (103), 25°C to 15°C day 4 (124), 15°C day 7 (102) and 25°C to 15°C day 7 (107). ***p hsp16.2p::GFP was measured as indicated. (F) Quantification of GFP levels. Number of biological replicates (n): 20°C day 1 (111), 35°C day 1 (102), 20°C day 2 (99), 35°C to 20°C day 2 (97), 20°C day 4 (121), 35°C to 20°C day 4 (114), 20°C day 6 (127) and 35°C to 20°C day 6 (106). ***p < 0.001 via Chi-square and Fisher’s exact tests. Scale bar = 100 μm.