Additional file 2. Amplification curves of NR-qPCR performed on field samples infected with P. vivax (n = 3; red), P. falciparum (n = 3; blue), P. malariae (n = 3; green), and P. brasilianum DNA (n = 1; orange). The amplification plots are shown for (A) Pvr47 and (B) Pfr364 assays
A simplified protocol for the identification of Plasmodium species by semi-nested multiplex polymera...
Table S1. Primers and probes used for amplification of 18S rRNA, pvs25 and pfs25, using qPCR or RT-q...
In epidemiological surveys and surveillance the application of molecular tools is essential in detec...
Additional file 4. Conditions of the Nested-PCR, R-qPCR, and NR-cPCR assays. The primers/probes used...
Additional file 5. Limit of detection (LOD) for (A) Pvr47 and (B) Pfr364 targets amplified by NR-qPC...
Additional file 2. Nested PCR targeting 18S rRNA with parasite genomic DNA (nD-PCR) and cDNA (nRT-PC...
Additional file 1. Primer sequences used to amplify hrp2 and P. falciparum, P. vivax, P. malariae an...
Molecular diagnosis of malaria offers many potential advantages over microscopy, including identific...
Additional file 4. Limits of detection (LOD) of RT-PCR and qRT-PCR for gametocyte detection. A. LOD ...
Additional file 11. Representative cycle threshold (Ct) values for Plasmodium falciparum 18S rRNA an...
A simplified protocol for the identification of Plasmodium species by semi-nested multiplex polymera...
A simplified protocol for the identification of Plasmodium species by semi-nested multiplex polymera...
Table S2. Reaction mixes for amplification of 18S rRNA, pvs25 and pfs25, using qPCR or RT-qPCR. The ...
Additional file 1. List of samples analysed: Combination of PURE-DNA extraction and LAMP-DNA amplifi...
The purpose of this work was to construct a screening method to detect the small subunit rRNA of mal...
A simplified protocol for the identification of Plasmodium species by semi-nested multiplex polymera...
Table S1. Primers and probes used for amplification of 18S rRNA, pvs25 and pfs25, using qPCR or RT-q...
In epidemiological surveys and surveillance the application of molecular tools is essential in detec...
Additional file 4. Conditions of the Nested-PCR, R-qPCR, and NR-cPCR assays. The primers/probes used...
Additional file 5. Limit of detection (LOD) for (A) Pvr47 and (B) Pfr364 targets amplified by NR-qPC...
Additional file 2. Nested PCR targeting 18S rRNA with parasite genomic DNA (nD-PCR) and cDNA (nRT-PC...
Additional file 1. Primer sequences used to amplify hrp2 and P. falciparum, P. vivax, P. malariae an...
Molecular diagnosis of malaria offers many potential advantages over microscopy, including identific...
Additional file 4. Limits of detection (LOD) of RT-PCR and qRT-PCR for gametocyte detection. A. LOD ...
Additional file 11. Representative cycle threshold (Ct) values for Plasmodium falciparum 18S rRNA an...
A simplified protocol for the identification of Plasmodium species by semi-nested multiplex polymera...
A simplified protocol for the identification of Plasmodium species by semi-nested multiplex polymera...
Table S2. Reaction mixes for amplification of 18S rRNA, pvs25 and pfs25, using qPCR or RT-qPCR. The ...
Additional file 1. List of samples analysed: Combination of PURE-DNA extraction and LAMP-DNA amplifi...
The purpose of this work was to construct a screening method to detect the small subunit rRNA of mal...
A simplified protocol for the identification of Plasmodium species by semi-nested multiplex polymera...
Table S1. Primers and probes used for amplification of 18S rRNA, pvs25 and pfs25, using qPCR or RT-q...
In epidemiological surveys and surveillance the application of molecular tools is essential in detec...