The CRISPR-Cas9 system is used for genome editing in mammalian cells by introducing double-strand breaks (DSBs) which are predominantly repaired via non-homologous end joining (NHEJ) or to lesser extent by homology-directed repair (HDR). To enhance HDR for improving the introduction of precise genetic modifications, we tested fusion proteins of Cas9 nuclease with HDR effectors to enforce their localization at DSBs. Using a traffic-light DSB repair reporter (TLR) system for the quantitative detection of HDR and NHEJ events in human HEK cells we found that Cas9 fusions with CtIP, Rad52, and Mre11, but not Rad51C promote HDR up to twofold in human cells and significantly reduce NHEJ events. We further compared, as an alternative to the direct ...
available in PMC 2014 March 30Targeted nucleases are powerful tools for mediating genome alteration ...
The discovery of Clustered Regions of Interspersed Palindromic Repeats (CRISPR), a defence system a...
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) is a ...
BACKGROUND: Precise genetic modifications are preferred products of CRISPR-Cas9 mediated gene editin...
CRISPR-associated (Cas) enzymes have revolutionized biology by enabling RNA-guided genome editing. H...
International audienceIn genome editing with CRISPR-Cas9, transgene integration often remains challe...
With the introduction of precision genome editing using CRISPR/Cas9 technology, we have entered a ne...
The CRISPR/Cas9 bacterial system has proven to be an powerful tool for genetic manipulation in sever...
The insertion of precise genetic modifications by genome editing tools such as CRISPR-Cas9 is limite...
The CRISPR/Cas9 system is a robust genome editing technology that works in human cells, animals and ...
The insertion of precise genetic modifications by genome editing tools such as CRISPR-Cas9 is limite...
Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA nicking or dou...
Precision CRISPR gene editing relies on the cellular homology-directed DNA repair (HDR) to introduce...
Methods to introduce targeted double-strand breaks (DSBs) into DNA enable precise genome editing by ...
SummaryCRISPR/Cas9 induces DNA double-strand breaks that are repaired by cell-autonomous repair path...
available in PMC 2014 March 30Targeted nucleases are powerful tools for mediating genome alteration ...
The discovery of Clustered Regions of Interspersed Palindromic Repeats (CRISPR), a defence system a...
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) is a ...
BACKGROUND: Precise genetic modifications are preferred products of CRISPR-Cas9 mediated gene editin...
CRISPR-associated (Cas) enzymes have revolutionized biology by enabling RNA-guided genome editing. H...
International audienceIn genome editing with CRISPR-Cas9, transgene integration often remains challe...
With the introduction of precision genome editing using CRISPR/Cas9 technology, we have entered a ne...
The CRISPR/Cas9 bacterial system has proven to be an powerful tool for genetic manipulation in sever...
The insertion of precise genetic modifications by genome editing tools such as CRISPR-Cas9 is limite...
The CRISPR/Cas9 system is a robust genome editing technology that works in human cells, animals and ...
The insertion of precise genetic modifications by genome editing tools such as CRISPR-Cas9 is limite...
Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA nicking or dou...
Precision CRISPR gene editing relies on the cellular homology-directed DNA repair (HDR) to introduce...
Methods to introduce targeted double-strand breaks (DSBs) into DNA enable precise genome editing by ...
SummaryCRISPR/Cas9 induces DNA double-strand breaks that are repaired by cell-autonomous repair path...
available in PMC 2014 March 30Targeted nucleases are powerful tools for mediating genome alteration ...
The discovery of Clustered Regions of Interspersed Palindromic Repeats (CRISPR), a defence system a...
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) is a ...