Impairment in locomotor coordination in <i>Amot fl/fl;Syn-Cre</i> and <i>Yap1 fl/fl;Syn-Cre</i> mice.

(A, B) General mobility in Amot fl/fl;Syn-Cre mice (n = 9) and control age- and sex-matched Amot fl/fl mice (n = 9) in the open field, showing the (A) time spent immobile (p = 0.6602) and (B) total distance traveled (p = 0.2787). (C, D) General mobility in Yap1 fl/fl;Syn-Cre mice (n = 6) and control age- and sex-matched Yap1 fl/fl mice (n = 8) in the open field, showing the (C) time spent immobile (p = 0.5806) and (D) total distance traveled (p = 0.2298). (E) Image from the rotarod experiment. (F) Locomotor coordination in Amot fl/fl mice (n = 10) and Amot fl/fl;Syn-Cre mice (n = 6) on P150, showing the latency to fall from the rotarod. p = 0.0092, p = 0.0022, p = 0.0051, p = 0.0422, and p = 0.0483. (G) Locomotor coordination in Yap1 fl/fl mice (n = 4) and Yap1 fl/fl;Syn-Cre mice (n = 6) on P150, showing the latency to fall from the rotarod. p = 0.3018, p = 0.1009, p = 0.0150, p = 0.0005, and p = 0.0416. (H) CatWalk gait analysis. The footprints of the right paws are shown in red, and the footprints of the left paws are shown in green. (I) Duty cycle in Amot fl/fl;Syn-Cre mice (n = 5) and Amot fl/fl (n = 11) control littermates. p = 0.9463 and p = 0.0401. (J) Duty cycle in Yap1 fl/fl;Syn-Cre (n = 6) and Yap1 fl/fl (n = 4) mice. p = 0.0538 and p = 0.0024. (K) Image from the foot-fault experiment. (L, M) Foot-fault analysis of Amot fl/fl (n = 7) and Amot fl/fl;Syn-Cre (n = 5) mice. (L) Time traveled. p = 0.0441. (M) Number of foot slips. p Yap1 fl/fl (n = 4) and Yap1 fl/fl;Syn-Cre (n = 6) mice. (N) Time traveled. p = 0.0271. (O) Number of foot slips. p = 0.0430. Numerical values that underlie the graphs are shown in S1 Data. Statistical significance was analyzed using two-tailed unpaired t test. *p p p < 0.001. Bars represent the mean ± SD in A, B, C, D, I, J, K, M, N, and O and mean ± SEM in F and G. Amot, angiomotin; BOS, base of support; FP, forepaws; HP, hind paws; ns, not significant; P, postnatal day; SD, standard deviation; SEM, standard error of the mean; Yap1, Yes-associated protein 1.