Effect of TNFα on ACE mRNA and protein expression in human endothelial cells.

(A) Time course of the effects of TNFα (10 ng/mL) on ACE mRNA levels in human umbilical vein endothelial cells relative to a triplet of housekeeping genes (HKG); n = 6 independent cell batches (two-way ANOVA/Bonferroni). (B) Time course of the effects of TNFα on ACE protein expression in human umbilical vein endothelial cells relative to non-muscle myosin heavy chain (NMM); n = 4–5 independent cell batches (one-way ANOVA/Newman-Keuls). (C&D) Expression of ACE mRNA (C) and protein (D) in human umbilical artery endothelial cells; n = 3 independent cell batches. ***P<0.001.