Quantitative FACS analysis of ATP1B2 patch I mutants.

Collated fluorescence data from directly and indirect exposure studies.

Kelechi Ugonna (540442)
Colin D. Bingle (233472)
Karen Plant (540443)
Kirsty Wilson (540444)
Mark L. Everard (540445)

March 2014

Cell infections and FACS analysis were performed as outlined in the methods section. The data rep...

β3 mutants of adducted residues.

Koichi Yuki (398264)
Weiming Bu (184333)
Motomu Shimaoka (398265)
Roderic Eckenhoff (398266)

April 2013

(A) PAC-1 binding to mock, αIIbβ3 wild type or mutant in 1 mM Mg2+/Ca2+ or ...

Single-cell genotyping results.

Alison M. Thompson (5165951)
Jordan L. Smith (610189)
Luke D. Monroe (5165948)
Jason E. Kreutz (1346787)
Thomas Schneider (252444)
Bryant S. Fujimoto (1349868)
Daniel T. Chiu (1313448)
Jerald P. Radich (445865)
Amy L. Paguirigan (1346784)

May 2018

(a) Scatterplots for each SD chip replicate containing OCI-AML3 cells are shown as 12 pane...

Quantitative FACS analysis of ATP1B2 patch II mutants.

Karolina Plössl (6659183)
Kristina Straub (6659186)
Verena Schmid (6659189)
Franziska Strunz (6659192)
Jens Wild (326687)
Rainer Merkl (3117)
Bernhard H. F. Weber (162289)
Ulrike Friedrich (6659195)

May 2019

Mean fluorescence intensity (MFI) from total cell population, calibrated against non-mutant ATP1B2. ...

Cell surface expression (CSE) of the mutant F proteins.

Wenyan Xie (702343)
Hongling Wen (787161)
Fulu Chu (787162)
Shaofeng Yan (706249)
Bin Lin (302181)
Wenli Xie (787163)
Ying Liu (18461)
Guijie Ren (787164)
Li Zhao (147498)
Yanyan Song (408130)
Chengxi Sun (787165)
Zhiyu Wang (282312)

August 2015

Cell surface expression was measured by flow cytometry with an anti-F monoclonal antibody followe...

Fbxo7 expression reduced the colony forming capacity and number of WT and p53 null cells.

Mikhail Lomonosov (349627)
El Kahina Meziane (349628)
Hongtao Ye (266214)
David E. Nelson (14786)
Suzanne J. Randle (349629)
Heike Laman (349630)

February 2013

(A) Representative FACS plots of retrovirally infected FL cells showing GFP expression. (<...

Knocking down NTPDase2 locally potentiates ATP-induced [Ca2+]i increase.

Chia-Lin Ho (259128)
Chih-Yung Yang (387657)
Wen-Jie Lin (387658)
Chi-Hung Lin (30220)

March 2013

(A) Fluorescence images showing how software-assisted cell demarcation and population identificat...

Fluorescence minus One (FMO) controls for the quantification of rare CPC events by FACS.

Yu-Xin Cui (335202)
Tom Johnson (335203)
Andreas Baumbach (335205)
Barnaby C. Reeves (335206)
Chris A. Rogers (335208)
Gianni D. Angelini (335210)
Debbie Marsden (335214)
Paolo Madeddu (247148)

February 2013

Isotype controls were prepared by the combination of all the individual fluorophore-conjugated is...

FACS analysis of HVT cell-derived MPs.

Liron Issman (501899)
Benjamin Brenner (501900)
Yeshayahu Talmon (501901)
Anat Aharon (501902)

December 2013

(a) MP size evaluation gate (R1) established using 0.78 µm beads with side scatter (SS) and forwa...

FACS analysis of the cellular binding activity of rP104-1-S/Fc and rP104-1-B/Fc.

Haiyan Gong (138159)
Kyousuke Kobayashi (334764)
Tatsuki Sugi (334765)
Hitoshi Takemae (334766)
Hitomi Kurokawa (334768)
Taisuke Horimoto (241790)
Hiroomi Akashi (334770)
Kentaro Kato (35147)

February 2013

A. Different types of cells were incubated with rP104-1-S/Fc before being stained with anti-Fc-FI...

Antigen and protein expression of human RBCs.

Slim Azouzi (500419)
Marc Gueroult (4162690)
Pierre Ripoche (367552)
Sandrine Genetet (367547)
Yves Colin Aronovicz (500420)
Caroline Le Van Kim (498410)
Catherine Etchebest (86630)
Isabelle Mouro-Chanteloup (367544)

December 2013

Values indicate the copy number of membrane proteins per red cell (×103), except for A...

Deep mutational scanning measurements of mutational impacts on folded RBD expression.

Tyler N. Starr (3276999)
Allison J. Greaney (11046384)
Cameron M. Stewart (14142067)
Alexandra C. Walls (11046345)
William W. Hannon (14142070)
David Veesler (11046387)
Jesse D. Bloom (8796629)

November 2022

(A) FACS scheme used for RBD expression deep mutational scanning assays on pooled Wuhan-Hu-1, Omicro...

EMS treatment increased the frequency of cells with extreme YFP fluorescence.

Jonathan D. Gruber (184335)
Kara Vogel (184338)
Gizem Kalay (184340)
Patricia J. Wittkopp (184345)

February 2012

(A) The relationship between “forward scatter” (“FSC”, a proxy for cell size) and YFP fluorescenc...

The expression of Fas increased on the surface of cells overexpressing 3OST-5.

Kazumi Hirano (144477)
Norihiko Sasaki (144478)
Tomomi Ichimiya (144479)
Taichi Miura (144480)
Toin H. Van Kuppevelt (5648701)
Shoko Nishihara (144481)

August 2012

(A) FACS analysis of cell surface Fas (left panel) or total Fas (right panel) in ce...

Quantitative data of single cell fluorescence measurements.

Paul C. M. Fogg (167770)
Alexander B. Westbye (310980)
J. Thomas Beatty (167775)

February 2013

Mean: Fluorescence in arbitrary units; FoP: Frequency of Parent; SEM: Standard Error of the Mean;...

Collated fluorescence data from directly and indirect exposure studies.

Kelechi Ugonna (540442)
Colin D. Bingle (233472)
Karen Plant (540443)
Kirsty Wilson (540444)
Mark L. Everard (540445)

March 2014

Cell infections and FACS analysis were performed as outlined in the methods section. The data rep...

β3 mutants of adducted residues.

Koichi Yuki (398264)
Weiming Bu (184333)
Motomu Shimaoka (398265)
Roderic Eckenhoff (398266)

April 2013

(A) PAC-1 binding to mock, αIIbβ3 wild type or mutant in 1 mM Mg2+/Ca2+ or ...

Single-cell genotyping results.

Alison M. Thompson (5165951)
Jordan L. Smith (610189)
Luke D. Monroe (5165948)
Jason E. Kreutz (1346787)
Thomas Schneider (252444)
Bryant S. Fujimoto (1349868)
Daniel T. Chiu (1313448)
Jerald P. Radich (445865)
Amy L. Paguirigan (1346784)

May 2018

(a) Scatterplots for each SD chip replicate containing OCI-AML3 cells are shown as 12 pane...

Quantitative FACS analysis of ATP1B2 patch II mutants.

Karolina Plössl (6659183)
Kristina Straub (6659186)
Verena Schmid (6659189)
Franziska Strunz (6659192)
Jens Wild (326687)
Rainer Merkl (3117)
Bernhard H. F. Weber (162289)
Ulrike Friedrich (6659195)

May 2019

Mean fluorescence intensity (MFI) from total cell population, calibrated against non-mutant ATP1B2. ...

Cell surface expression (CSE) of the mutant F proteins.

Wenyan Xie (702343)
Hongling Wen (787161)
Fulu Chu (787162)
Shaofeng Yan (706249)
Bin Lin (302181)
Wenli Xie (787163)
Ying Liu (18461)
Guijie Ren (787164)
Li Zhao (147498)
Yanyan Song (408130)
Chengxi Sun (787165)
Zhiyu Wang (282312)

August 2015

Cell surface expression was measured by flow cytometry with an anti-F monoclonal antibody followe...

Fbxo7 expression reduced the colony forming capacity and number of WT and p53 null cells.

Mikhail Lomonosov (349627)
El Kahina Meziane (349628)
Hongtao Ye (266214)
David E. Nelson (14786)
Suzanne J. Randle (349629)
Heike Laman (349630)

February 2013

(A) Representative FACS plots of retrovirally infected FL cells showing GFP expression. (<...

Knocking down NTPDase2 locally potentiates ATP-induced [Ca2+]i increase.

Chia-Lin Ho (259128)
Chih-Yung Yang (387657)
Wen-Jie Lin (387658)
Chi-Hung Lin (30220)

March 2013

(A) Fluorescence images showing how software-assisted cell demarcation and population identificat...

Fluorescence minus One (FMO) controls for the quantification of rare CPC events by FACS.

Yu-Xin Cui (335202)
Tom Johnson (335203)
Andreas Baumbach (335205)
Barnaby C. Reeves (335206)
Chris A. Rogers (335208)
Gianni D. Angelini (335210)
Debbie Marsden (335214)
Paolo Madeddu (247148)

February 2013

Isotype controls were prepared by the combination of all the individual fluorophore-conjugated is...

FACS analysis of HVT cell-derived MPs.

Liron Issman (501899)
Benjamin Brenner (501900)
Yeshayahu Talmon (501901)
Anat Aharon (501902)

December 2013

(a) MP size evaluation gate (R1) established using 0.78 µm beads with side scatter (SS) and forwa...

FACS analysis of the cellular binding activity of rP104-1-S/Fc and rP104-1-B/Fc.

Haiyan Gong (138159)
Kyousuke Kobayashi (334764)
Tatsuki Sugi (334765)
Hitoshi Takemae (334766)
Hitomi Kurokawa (334768)
Taisuke Horimoto (241790)
Hiroomi Akashi (334770)
Kentaro Kato (35147)

February 2013

A. Different types of cells were incubated with rP104-1-S/Fc before being stained with anti-Fc-FI...

Antigen and protein expression of human RBCs.

Slim Azouzi (500419)
Marc Gueroult (4162690)
Pierre Ripoche (367552)
Sandrine Genetet (367547)
Yves Colin Aronovicz (500420)
Caroline Le Van Kim (498410)
Catherine Etchebest (86630)
Isabelle Mouro-Chanteloup (367544)

December 2013

Values indicate the copy number of membrane proteins per red cell (×103), except for A...

Deep mutational scanning measurements of mutational impacts on folded RBD expression.

Tyler N. Starr (3276999)
Allison J. Greaney (11046384)
Cameron M. Stewart (14142067)
Alexandra C. Walls (11046345)
William W. Hannon (14142070)
David Veesler (11046387)
Jesse D. Bloom (8796629)

November 2022

(A) FACS scheme used for RBD expression deep mutational scanning assays on pooled Wuhan-Hu-1, Omicro...

EMS treatment increased the frequency of cells with extreme YFP fluorescence.

Jonathan D. Gruber (184335)
Kara Vogel (184338)
Gizem Kalay (184340)
Patricia J. Wittkopp (184345)

February 2012

(A) The relationship between “forward scatter” (“FSC”, a proxy for cell size) and YFP fluorescenc...

The expression of Fas increased on the surface of cells overexpressing 3OST-5.

Kazumi Hirano (144477)
Norihiko Sasaki (144478)
Tomomi Ichimiya (144479)
Taichi Miura (144480)
Toin H. Van Kuppevelt (5648701)
Shoko Nishihara (144481)

August 2012

(A) FACS analysis of cell surface Fas (left panel) or total Fas (right panel) in ce...

Quantitative data of single cell fluorescence measurements.

Paul C. M. Fogg (167770)
Alexander B. Westbye (310980)
J. Thomas Beatty (167775)

February 2013

Mean: Fluorescence in arbitrary units; FoP: Frequency of Parent; SEM: Standard Error of the Mean;...

Collated fluorescence data from directly and indirect exposure studies.

Kelechi Ugonna (540442)
Colin D. Bingle (233472)
Karen Plant (540443)
Kirsty Wilson (540444)
Mark L. Everard (540445)

March 2014

Cell infections and FACS analysis were performed as outlined in the methods section. The data rep...

β3 mutants of adducted residues.

Koichi Yuki (398264)
Weiming Bu (184333)
Motomu Shimaoka (398265)
Roderic Eckenhoff (398266)

April 2013

(A) PAC-1 binding to mock, αIIbβ3 wild type or mutant in 1 mM Mg2+/Ca2+ or ...

Single-cell genotyping results.

Alison M. Thompson (5165951)
Jordan L. Smith (610189)
Luke D. Monroe (5165948)
Jason E. Kreutz (1346787)
Thomas Schneider (252444)
Bryant S. Fujimoto (1349868)
Daniel T. Chiu (1313448)
Jerald P. Radich (445865)
Amy L. Paguirigan (1346784)

May 2018

(a) Scatterplots for each SD chip replicate containing OCI-AML3 cells are shown as 12 pane...

Quantitative FACS analysis of ATP1B2 patch I mutants.

Abstract

Extracted data

Quantitative FACS analysis of ATP1B2 patch I mutants.

Abstract

Extracted data

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