Cytokine profile of 15-day old Balb/c mice infected via the rear footpad with 2.57 x 10⁶ PFU.

Serum was collected 3, 10, 15 and 30 days post-infection for analysis of soluble factor levels. The levels of MCP-1 (A), TNF (B), INF-γ (C) and IL-6 (D) were elevated on day 3 PI. At day 10, TNF (B) and INF-γ (C) remained high and the level of IL-10 (E) was elevated. At day 30 PI TNF (B) and IL-4 (F) were present. Statistics were performed with one-way ANOVA, Bonferroni’s multiple comparisons test. Gray bars: Infected. Black bars: negative control. (* P value ≤ 0.05; ** P value ≤ 0.01; **** P value ≤ 0.0001). N = 3 animals/group.