MOESM1 of Expression of benzoyl-CoA metabolism genes in the lignocellulolytic host Caldicellulosiruptor bescii

Additional file 1: Figure S1. Diagram of plasmid pJOT1 used to heterologously express a codon-optimized Ferp_1044 gene from Ferroglobus placidus, encoding a benzoyl-CoA ligase, in C. bescii. Figure S2. Diagram of plasmid pJOT2 used to heterologously express codon-optimized genes from Ferroglobus placidus encoding a benzoyl-CoA ligase (Ferp_1044), a benzoyl-CoA reductase (Ferp_1184–Ferp_1187), and a putative benzoate transporter (Ferp_1370). Unique ribosome binding site regions (~ 40 upstream basepairs) were identified from highly transcribed C. bescii genes (Blumer-Schuette et al. 2012) and placed upstream of each gene. Table S1. Primers used in this study. Table S2. Plasmids used in this study