Oct1 restricts tumorigenicity in a model driven by heterozygous <i>Apc</i> deletion in Lrig1⁺ cells.

(A) Example distal colons are shown of an allelic series of Oct1+/+, Oct1fl/+ and Oct1fl/fl animals that were additionally Apcfl/+ and Lrig1-CreERT2. (B) Quantification of colon tumor incidence across multiple animals. Oct1+/+ N = 14, Oct1fl/+ N = 12 and Oct1fl/fl N = 6. (C) Representative H&E, and Oct1 and β-catenin IHC of colon tumor tissue and adjacent GU tissue from Apcfl/+;Lrig1-CreERT2 and Apcfl/+;Oct1fl/fl;Lrig1-CreERT2 animals. Mice were analyzed 100 days after tamoxifen treatment and Apc/Oct1 deletion. (D) Tumor mitoses were studied using pH3 IHC. (E) Quantification of pH3 staining events in tumor and GU tissue from 3 mice per group (10 fields per animal). (F) Quantification of tumor grade across multiple animals. Oct1+/+ N = 14, Oct1fl/+ N = 12 and Oct1fl/fl N = 6. Tumor grade was low in all cases. (G) Similar analysis performed for tumor area.