Additional file 2: of Anti-EMT properties of CoQ0 attributed to PI3K/AKT/NFKB/MMP-9 signaling pathway through ROS-mediated apoptosis

CoQ0 treatment induces G2/ M cell-cycle arrest in MDA-MB-231 cells. (a) Cells were treated with CoQ0 (5–15 μM) for 24 h, stained with PI and analyzed for cell-cycle phase using flow cytometry. The cellular distributions (%) in different phases of the cell cycle (sub-G1, G1,S and G2/M) were determined after treatment with AS. The flow cytometry graph shown here is from one representative experiment that was performed in triplicates. (b) The effects of CoQ0 AS on cell-cycle regulatory proteins. HL-60 cells were treated with increasing concentrations of CoQ0 (5–15 μM) for 24 h. Cell-cycle regulatory proteins, including Cyclin A, Cyclin B, p21, Cdc2, Cdc25C, CDK2, and CDK4 were examined using Western blot analyses. The results are presented as the mean ± SD of three independent assays. **p < 0.05, ***p < 0.001 significant compared to control cells. (PPTX 4253 kb