Vessel iluminated alternatively in bright-field and dark-field illumination surrounded by capillaries

Image sequence showing a vessel laying on the nerve fiber layer of a healthy subject. The vessel is partly illuminated in bright-field and dark-field configurations. The dark field in the center was generated using a field diaphragm in the illumination path. A bed of capillaries surround the vessel. Acquisition frequency is 200Hz and visualization frequency is 50Hz. Scale bar is 50µm.Image sequence was corrected of the inhomogenous background and registered