Caspase 3/7 activities correspond with the generation of 30–100 kb fragments in response to SN38 treatment.

(A) Caspase 3/7 activity (left panel) in H1299 cells (parental and HMGA2 KO) after SN38 treatment at indicated doses for 24 h and cell survival (CCK8) assay (right panel) with H1299 and HMGA2 KO cells after treatment with SN38 for 48 h (n = 3 independent experiments with 3 technical replicates in each). Mean of untreated triplicates used for normalization. Error bars show SD. Unpaired two-tailed t-tests. ** p (B) Caspase 3/7 activity in A549 cells (parental and three recombinant human HMGA2-expressing cell lines) after SN38 treatment at indicated doses for 24 h (n = 3 independent experiments with 3 technical replicates in each). Mean of untreated triplicates used for normalization. Error bars show SD. Unpaired two-tailed t-tests. **** p (C) Caspase 3/7 activity (top panel) in HeLa cells (parental and three recombinant human HMGA2-expressing cell lines) after SN38 treatment at indicated doses for 24 h and cell survival (CCK8) assay (bottom panel) with HeLa and P2 cells (recombinant HMGA2 expressing cell line) after treatment with SN38 for 48 h (n = 3 independent experiments with 3 technical replicates in each). Mean of untreated triplicates used for normalization. Error bars show SD. Unpaired two-tailed t-tests. *** p (D) Caspase 3/7 activity in HT1080 C2 cells (Dox-/+) after SN38 treatment at indicated doses for 24 h (n = 2 independent experiments with 3 technical replicates in each). Mean of untreated triplicates used for normalization. Error bars show SD. Unpaired two-tailed t-tests. *** p < 0.001, **** p < 0.0001.