A. Schematic representation of the triple-reporter system for direct detection of clustered mutations in ssDNA. Subtelomeric reporter: CAN1, URA3, and ADE2 genes were placed within disrupted LYS2 gene, close to de novo constructed telomere at Chromosome V in the cdc13-1ts mutant strain. At 37°C, uncapping of telomeres and prevention of resynthesis of the resected telomeres in cdc13-1ts cells results in persistent ssDNA comprising the reporter genes up to 6 hours without significant loss of viability. Simultaneous mutations in CAN1 (blue vertical arrows) and ADE2 (red vertical arrows) give rise to CanR Red colonies. Midchromosome reporter: CAN1, URA3, and ADE2 genes were placed in the middle of the right arm of Chromosome II, more than 300 k...
Hydrogen peroxide is an oxidizing agent which can be generated intracellularly either during normal ...
The major DNA repair pathways operate on damage in double-strand DNA because they use the intact str...
Mutations are typically perceived as random, independent events. We describe here non-random cluster...
<p>(A) Three reporter genes, <i>ADE2</i>, <i>URA3</i>, and <i>CAN1</i>, were relocated from their re...
Chromosomal DNA must be in single-strand form for important transactions such as replication, transc...
<div><p>Chromosomal DNA must be in single-strand form for important transactions such as replication...
Mutation frequency measurement experiments were set up in such a way that each individual culture ha...
A. Spectra of mutations identified by Sanger sequencing of CanR Red isolates in the triple subtelome...
A. Fractions of the mutations at C in specific trinucleotide motifs among all of the substitutions d...
A. Frequency of spontaneous and peroxide-induced CanR mutations and CanR Red closely spaced mutation...
This is an open-access article distributed under the terms of the Creative Commons Public Domain dec...
(A) Experimental protocol to study strand specific (G-rich or C-rich) breaks/gaps on telomere is sho...
PhD ThesisTelomeres are bound by multiple ‘capping’ proteins, which protect chromosome ends from fu...
Genome instability continuously presents perils of cancer, genetic disease and death of a cell or an...
Single-stranded DNA (ssDNA) viruses mutate and evolve at similar rates to RNA viruses, and more rapi...
Hydrogen peroxide is an oxidizing agent which can be generated intracellularly either during normal ...
The major DNA repair pathways operate on damage in double-strand DNA because they use the intact str...
Mutations are typically perceived as random, independent events. We describe here non-random cluster...
<p>(A) Three reporter genes, <i>ADE2</i>, <i>URA3</i>, and <i>CAN1</i>, were relocated from their re...
Chromosomal DNA must be in single-strand form for important transactions such as replication, transc...
<div><p>Chromosomal DNA must be in single-strand form for important transactions such as replication...
Mutation frequency measurement experiments were set up in such a way that each individual culture ha...
A. Spectra of mutations identified by Sanger sequencing of CanR Red isolates in the triple subtelome...
A. Fractions of the mutations at C in specific trinucleotide motifs among all of the substitutions d...
A. Frequency of spontaneous and peroxide-induced CanR mutations and CanR Red closely spaced mutation...
This is an open-access article distributed under the terms of the Creative Commons Public Domain dec...
(A) Experimental protocol to study strand specific (G-rich or C-rich) breaks/gaps on telomere is sho...
PhD ThesisTelomeres are bound by multiple ‘capping’ proteins, which protect chromosome ends from fu...
Genome instability continuously presents perils of cancer, genetic disease and death of a cell or an...
Single-stranded DNA (ssDNA) viruses mutate and evolve at similar rates to RNA viruses, and more rapi...
Hydrogen peroxide is an oxidizing agent which can be generated intracellularly either during normal ...
The major DNA repair pathways operate on damage in double-strand DNA because they use the intact str...
Mutations are typically perceived as random, independent events. We describe here non-random cluster...