Additional file 1: of Characterization of the molecular changes associated with the overexpression of a novel epithelial cadherin splice variant mRNA in a breast cancer model using proteomics and bioinformatics approaches: identification of changes in cell metabolism and an increased expression of lactate dehydrogenase B

Figure S1. E-cadherin protein expression analysis in MCF7Ecadvar and MCF7pcDNA3 cells. Western immunoblotting of the 120 kDa E-cadherin wild type (E-cadherin wt) full length form in total protein extracts obtained from 3 MCF7pcDNA3 (1 to 3, 10 μg per lane) and 3 MCF7Ecadvar (4 to 6, 20 μg per lane) cell cultures. The immunodetection of β-tubulin (55 kDa) was used as loaded control. (TIF 2449 kb