Comparison of sensitivity between the miniPCR and Maxygen thermal cyclers.

A) Sensitivity trials using a 91 bp amplicon in miniPCR (left section) and Maxygen (right section) thermal cyclers. The initial template amount was gradually increased from left to right: 66 (lane 1), 6.62 x 102 (lane 2), 6.62 x 104 (lanes 3), 6.62 x 106 (lane 4), 6.62 x 107 (lane 5), 1.30 x 108 (lane 6), and 3.9 x 108 DNA copies (lane 7), and negative control (lane 8). B). Sensitivity trials using a 300bp amplicon in the miniPCR (left section) and Maxygen (right section) thermal cyclers. The initial template amount was gradually increased from left to right: negative control (lane 1), 66 (lane 2), 6.62 x 102 (lane 3), 6.62 x 104 (lanes 4), 6.62 x 106 (lane 5), 6.62 x 107 (lane 6), 1.30 x 108 (lane 7), and 3.9 x 108 DNA copies (lane 8). These results showed no significant differences between devices. (C) Sensitivity trials using a 252bp Zika virus amplicon in the miniPCR (left section) and Maxygen (right section) thermal cyclers. The initial template amount was gradually increased from left to right: 66 (lane 1), 6.62 x 102 (lane 2), 6.62 x 104 (lane 3), 6.62 x 106 (lane 4), 6.62 x 107 (lane 5), 1.30 x 108 (lane 6), and 3.9 x 108 DNA copies (lane 7), and negative control (lane 8).