Comparing cell line DNA copy number to RNA expression for tRNApyl and pylRSwt assays.

Copy numbers of pylRS and tRNA were determined in six amber suppression competent cell lines. (A) The calculated DNA copy numbers plotted relative to the corresponding RNA expression levels. A loose correlation between copy number and expression is observed for pylRSwt; however, tRNA expression levels do not seem to depend on copy number. (B) The GFP:RFP ratios plotted relative to corresponding RNA expression levels.