Degree of exposure of Trp residues.

Fluorescence emission spectra of 5G4 mAb (A) and Herceptin (B) attenuated with different concentrations of acrylamide are shown. Black, red, light blue, yellow, dark blue, brown and pink colors spectra correspond to 0, 50, 100, 150, 250, 300, 350 and 400 mmol/L acrylamide. C and D: Stern Volmer representation for acrylamide attenuated 5G4 mAb and Herceptin. Different batches of each mAb are shown. 5G4 mAb batch 1 (black), 5G4 mAb batch 2 (red), 5G4 mAb batch 3 (blue), Herceptin batch 1 (green) and Herceptin batch 2 (pink).