Intrinsic Rbpj in T cells is required for TCRαβ⁺CD8αα⁺ IELs.

Bone marrow cells (1.5 × 107) and a 1:1 ratio from Rbpj−/− (CD45.2) and Rbpj+/+ (CD45.1) mice were transplanted into irradiated (9.5 Gy) C57BL/6 mice (CD45.1+CD45.2+). (A) The ratio of intraepithelial CD45+ cells between Rbpj−/− and Rbpj+/+ mice. (B) After 6 wk posttransplantation, the number and frequency of TCRαβ+CD8αα+ IELs from Rbpj−/− (CD45.2) and Rbpj+/+ (CD45.1) mice were evaluated. The data are shown as mean ± S.D., and *** indicates p n = 5 mice in each experiment. Data associated with this figure can be found in the supplemental data file (S1 Data). IEL, intraepithelial lymphocyte; N.S.; not significant; Rbpj, recombination signal binding protein for immunoglobulin kappa J region; TCR, T-cell receptor.